NAD+ research overview
NAD+ (beta-nicotinamide adenine dinucleotide, oxidized form) is a dinucleotide coenzyme, not a peptide: a nicotinamide ribonucleotide and an adenosine ribonucleotide joined through a pyrophosphate bridge. It is present in all living cells, where it accepts electrons in redox reactions (becoming NADH) and serves as a substrate for enzymes such as sirtuins, PARPs and CD38.
NAD+ supplied by Pep Shop Texas is a lyophilized powder for laboratory research only. It is not sold for human or veterinary use.
Identity and structure
| Name | NAD+ (beta-nicotinamide adenine dinucleotide, oxidized form; beta-NAD) |
|---|---|
| Class | Dinucleotide coenzyme (two nucleotides linked by a pyrophosphate group) |
| Molecular formula | C21H27N7O14P2 |
| Molecular weight | about 663.4 g/mol |
| CAS number | 53-84-9 |
| Reduced form | NADH |
| Form | White to off-white lyophilized powder in a sealed vial |
Published research
NAD+ has been studied for decades in enzymology, yeast genetics and rodent models, and laboratories measure it with LC-MS and enzymatic cycling assays. The papers below are listed by what was measured. They are not evidence of safety or effect in people.
Purified yeast and mouse Sir2 proteins in vitro, plus yeast strains. Measured histone deacetylase activity with and without NAD+, and which histone lysine residues were deacetylated.
Imai S, Armstrong CM, Kaeberlein M, Guarente L. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature. 2000;403(6771):795-800. PubMed 10693811
Tissues from young and old wild-type and CD38-knockout mice. Measured tissue NAD+ levels, CD38 expression and enzyme activity, SIRT3 activity and mitochondrial respiration.
Camacho-Pereira J, Tarragó MG, Chini CCS, et al. CD38 dictates age-related NAD decline and mitochondrial dysfunction through an SIRT3-dependent mechanism. Cell Metab. 2016;23(6):1127-1139. PubMed 27304511
Analytical method paper. Describes liquid chromatography-mass spectrometry with internal standards for quantifying NAD+ and related metabolites in cells and tissues.
Trammell SA, Brenner C. Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites. Comput Struct Biotechnol J. 2013;4:e201301012. PubMed 24688693
Storage of lyophilized material
Keep lyophilized NAD+ sealed, dry and away from light; the powder takes up moisture from air. Supplier data sheets give -20 °C (a standard freezer) for long-term storage. Let a cold vial reach room temperature before opening it so moisture does not condense on the powder.
Lab testing
Each batch Pep Shop Texas sells is sent to an independent lab for identity (LC-MS) and purity (HPLC) testing. How we test.
NAD+: strengths, price and stock · All lab reports · A–Z index · Full collection
For laboratory research use only. Not for human or veterinary use. Not a drug, food, cosmetic or supplement. Pep Shop Texas is not a compounding pharmacy and does not give dosing or preparation guidance. Nothing on this page is medical advice or a claim about use in people.